国产青榴社区91精品,久久成人精品免费播放,久久精品人人做人人试看

Home>(Research) Service>Transcriptome & Epigenome Sequencing>Transcriptome>Eukaryotic mRNA Sequencing with Reference Genome

Eukaryotic mRNA Sequencing with Reference Genome

FAQ

  •  A brief introduction of the construction of eukaryotic transcriptome library


    1) mRNA was separated by magnetic beads for polyA structure of mRNA

    2) mRNA fragmentation

    3) The base T is replaced by U during the second strand of cDNA synthetization to achieve the purpose of strand specific library.

    4) 5 'end repair, 3' end add “A”

    5) Add adapters

    6) Collect fragments with specific length, generally 300bp

    7) PCR

    8) Sequencing


  • How to select appropriate genes for qRT-PCR verification?


     Firstly, according to the enrichment results of GO/KEGG, the genes concerned shall be selected. Then, according to the results of differential expression analysis, the genes with higher fold change value, smaller P value and higher FPKM value shall be verified by qRT-PCR.





主站蜘蛛池模板：
酉阳|
桂平市|
浦东新区|
永寿县|
渭南市|
泸溪县|
双辽市|
扎鲁特旗|
元阳县|
万全县|
卓尼县|
南陵县|
法库县|
密云县|
勐海县|
稷山县|
台山市|
佛教|
于都县|
宝鸡市|
华阴市|
荔浦县|
阜南县|
商南县|
白水县|
留坝县|
广平县|
青铜峡市|
贡嘎县|
临沂市|
玉溪市|
灌南县|
军事|
香格里拉县|
雅安市|
洪泽县|
鄂州市|
资中县|
福贡县|
射洪县|
阿拉善左旗|