CircRNA Sequencing
Circular RNA is a kind of noncoding RNA molecules without 5 'end cap and 3' end poly (A) tail, which forms a stable closed ring structure by covalent bond. CircRNA widely exists in biological cells, which plays an important role in regulating miRNA and gene expression. With the development of high-throughput sequencing technology, many circRNA have been identified in eukaryotes. Based on high-throughput sequencing technology, the identification, expression abundance and structure of circRNA in biological samples are analyzed, which accelerates further gene scientific research of circRNA.
Project Advice
Most circRNA sequencing researches focus on different comparative groups comparison analysis, such as control vs case. If the space-time factors such as different growth and development stages or the whole process of disease occurrence and development are considered, multiple comparisons will be analyzed to obtain the significantly different cricRNAs. Then the source genes of circRNAs are predicted and their enrichment analysis are carried out. At least 6 biological repeats are recommended for cricRNA sequencing.
