RNA methylation refers to the methylation occurred at different RNA positions. The common post transcriptional modifications of RNA include N6-methyladenine (m6A) and C5-methylcytidine (m5C). Recent studies have shown that m6A modification plays an important role in regulating gene expression, splicing, RNA editing, RNA stability, controlling mRNA longevity and degradation, and mediating circular RNA translation. Therefore, Methylation RNA immunoprecipitation combined with high-throughput sequencing (MeRIP-seq) facilitates to solve the biological problems such as cell differentiation, biological development, disease occurrence and development, heat shock response and so on. It can be used to comprehensively study the post transcriptional methylation modification profile of RNA, which is the key technology of epigenetics research.
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Test group VS control group, 3-5 biological repeats recommended for each group
Comparison within group: IP group VS input group (input group is required for identifying the specific combination of IP group with antibody)
Sequencing mode: PE150
Data amount: 6G
