国产青榴社区91精品,久久成人精品免费播放,久久精品人人做人人试看

Home>(Research) Service>Transcriptome & Epigenome Sequencing>Transcriptome>Full Length Transcriptome Sequencing

Full Length Transcriptome Sequencing

FAQ

  • Why do full-length transcriptome recommend "3+2"?


    So far most of the reported full length transcriptome articles adopt the mode of "3+2". “3” refers to the third generation sequencing that detects more accurate structural variation. For the species with reference genome, it can accurately detect alternative splicing, fusion genes, and predict new genes. “2” refers to the second generation sequencing that achieve more accurate quantification than the third generation sequencing. For the species without reference genome, “3+2” sequencing strategy can provide accurate reference sequences, which is helpful for subsequent differential expression analysis. In addition, the "3+2" sequencing strategy can use the data of the second generation sequencing to correct the third generation sequencing data.


  •  Does the full-length transcriptome sequencing library need to be fragmented and assembled?


    The full-length transcriptome sequencing is based on the PacBio sequencing platform, which can directly obtain the complete transcripts containing 5 ', 3'UTR, polyA tail. It overcomes the limitations of short assembly and incomplete information of transcripts of the species without reference genome.

     


  • How to choose the data amount of full-length transcriptome sequencing?


    Regarding to PacBio Sequel platform, it is recommended to have 40G sequencing data amount. If low expression genes are interested, then 60G even more data amount are suggested.


  • What species are suitable for full-length transcriptome sequencing?


    Both species with / without reference genomes are suitable.


  • How to prepare the right sample for full-length transcriptome sequencing?


    It mainly depends on the research purpose. In general, if you want to get more comprehensive transcripts of a species, it is recommended to collect different tissue samples of this species to extract RNA, mixed RNA to get as comprehensive transcripts as possible. For example, roots, stems, leaves, flowers, fruits of plants; liver, kidney, intestine, skin, and other parts of animals are recommended to collect. If full-length transcriptome sequencing is for a certain tissue part, then different development stages of the tissue can be sampled.

     


主站蜘蛛池模板: 德阳市| 灵川县| 大新县| 黑水县| 和硕县| 康保县| 齐河县| 沙坪坝区| 洛隆县| 安新县| 镇原县| 福清市| 天峨县| 葵青区| 永昌县| 天柱县| 山东省| 牟定县| 莱阳市| 信丰县| 米脂县| 成武县| 襄汾县| 余庆县| 阿巴嘎旗| 通道| 新绛县| 永定县| 仪征市| 神农架林区| 三门县| 西乌| 白山市| 藁城市| 班玛县| 济宁市| 休宁县| 台北市| 霍邱县| 罗田县| 高安市|