2019-06-07
Abstract
Anaerobic ammonium oxidation (anammox) is an important process in marine nitrogen cycle. In thisstudy, diverse anammox bacteria were identified in the sediments of the Changjiang (Yangtze) Estuaryand its adjacent area. Specifically, the community characters of anammox bacteria in the studied areawere studied by quantitative polymerase chain reaction (qPCR), as well as 16S rRNA gene- and functionalgene (hzo)-based Roche 454 sequencing. The abundance of denitrifying bacteria detected by the nirS genewas greater than that of anammox bacteria. 16S rRNA and hzo gene fragments affiliating with knownanammox bacterial lineages were recovered, and the two major phylotypes belonged to the CandidatusScalindua (Ca. Scalindua) genus, with >90% sequence similarity. A phylogenetic analysis detected theScalindua and Brocadia genera together with some anammox-like bacterial clusters, which suggested ahigher diversity in the studied ecosystem than in open ocean environment, where only Scalindua genuswas detected. A redundancy analysis (RDA) showed that total organic carbon (TOC) and total nitrogen (TN)content in sediments significantly influenced anammox bacterial abundance of. Spearman correlationanalyses confirmed that the spatial variation in anammox bacterial abundance was highly correlatedwith TOC (P < 0.01) and TN (P < 0.01) contents in sediments.
Text link：https://www.sciencedirect.com/science/article/abs/pii/S0723202018304107
