2018-10-30
Background: Systemic lupus erythematous (SLE) is an autoimmune disease characterized by production of autoantibodies directed against various autoantigens. But the expression profiles and functions of circRNAs in SLE are still scarce. Objectives: To explore the roles of circRNA in SLE and its potential diagnostic potential in SLE. Methods: SLE patients and healthy control subjects were recruited. CD4+ T cellswere isolated, circRNA microarray analysis were used to screen for circRNA candidate in CD4+ T cells. Expression of DNMT1, CD11a and CD70 and methylation level of CD11a and CD70 were detected after transfecting hsa_circ_0012919-targeted siRNA. The network analysis of hsa_circ_0012919 was used by bioinformatics. Luciferase reporter assay and FISH assay were used for screen for which miRNAs could bind with hsa_circ_0012919. Results: 12 circRNAs were upregulated and 2 circRNAs were downregulated in SLE patients group after circRNA microarray analysis. Hsa_circ_0012919 was further confirmed to be significantly different between healthy control and SLE patients (p<0.05) and associated with SLE characters (p<0.05). Downregulation of hsa_circ_0012919 1) increased the expression of DNMT1 and reduced the expression of CD70, CD11a, 2)reversed the DNA hypomethylation of CD11a and CD70 in CD4+T cells of systemic lupus erythematous, but it could be reversed by downregulation of DNMT1. Hsa_circ_0012919 regulated KLF13 and RANTES by miRNA-125a. Conclusion:Hsa_circ_0012919 could be regarded as a biomarker for SLE and hsa_circ_0012919
text link ：http://www.clinsci.org/content/early/2018/09/20/CS20180403.long
