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Bacterial community evolutions driven by organic matter and powder activated carbon in simultaneous anammox and denitrification (SAD) process

2018-06-21

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Abstract


A distinct shift of bacterial community driven by organic matter (OM) and powder activated carbon (PAC) was discovered in thesimultaneous anammox and denitrification (SAD) process which was operated in an antifoulingsubmerged anaerobic membrane bio-reactor. Based on anammox performance, optimal OM dose (50 mg/L) was advised to start up SAD process successfully. The results of qPCR and high throughput sequencing analysisindicated that OM played a key role in microbial community evolutions, impelling denitrifiers to challengeanammox’s dominance. The addition of PAC not only mitigated the membrane fouling, but also stimulated the enrichment of denitrifiers, accounting for the predominant phylum changing from Planctomycetes toProteobacteria in SAD process. Functional genes forecasts based on KEGG database and COG database showed that the expressions of full denitrification functional genes were highly promoted in RC, which demonstrated the enhanced full denitrification pathway driven by OM and PAC under low COD/N value (0.11).


Introduction


Recently, nitrogen-rich wastewater has received much attention due to its potential threat to aquatic ecosystem. Anaerobic ammonium

oxidation (anammox) was regarded as a high efficient and cost saving process to remove nitrogen from wastewater. In this process, anammox bacteria directly convert ammonium to nitrogen gas using nitrite as electron acceptor under anaerobic condition, as shown in Eq. (1) (Strous et al., 1998).


text linkhttps://www.sciencedirect.com/science/article/pii/S0960852417321508



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